Growth and metabolism of Pneumocystis carinii in axenic culture
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چکیده
منابع مشابه
Growth of Pneumocystis Carinii in Axenic Culture
SUMMARY In this research, which was done for two years, we investigated various liquid and solid media without feeder ceells, for the ability to support growth of P. carinii. Data obtained from counting the organisms nuclei showed that, addition of Cycloheximide, penicillin, & streptomycin to neopeptoneN-Acetyle Glucoseamine (N.P.G. medium) can provides growth requirements of organism in its...
متن کاملContinuous axenic cultivation of Pneumocystis carinii.
Continuous axenic culture of Pneumocystis carinii has been achieved. A culture vessel is used that allows for frequent medium exchange without disturbance of organisms that grow attached to a collagen-coated porous membrane. The growth medium is based on Minimal Essential Medium with Earle's salt supplemented with S-adenosyl-L-methionine, putrescine, ferric pyrophosphate, N-acetyl glucosamine, ...
متن کاملActivity of inosine analogs against Pneumocystis carinii in culture.
Three analogs of inosine, formycin B, allopurinol ribonucleoside, and 9-deazainosine, were tested for their ability to suppress proliferation of Pneumocystis carinii in culture with WI-38 cells. The organism was inhibited by 9-deazainosine at 10 micrograms/ml, and there was some inhibition at 1 microgram/ml. Formycin B was effective only at 40 micrograms/ml. Allopurinol ribonucleoside had littl...
متن کاملPropagation of Pneumocystis carinii in Vero cell culture.
Pneumocystis carinii derived from infected murine lung was found to be capable of limited growth in Vero African green monkey kidney cell cultures. The observed increase in the number of cyst forms was influenced by the ratio of cysts to host cells in the inocula, duration of passage, and formulation of the cell culture media. Maximum growth was achieved by inoculating 1.3 X 10(5) cysts per 75-...
متن کاملBiological profile and response to anti-pneumocystis agents of Pneumocystis carinii in cell culture.
Although the growth characteristics of Pneumocystis carinii have been described in several cell culture systems, the response of this organism to the drugs of choice, trimethoprim-sulfamethoxazole and pentamidine isethionate, have not been described in vitro. The effect of various concentrations of drugs against P. carinii on the growth of this potentially hazardous opportunistic organism as we...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1990
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.28.6.1385-1394.1990